Flow Cytometry: A Novel Approach for Indirect Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies
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flow cytometry: a novel approach for indirect assessment of protamine deficiency by cma3 staining, taking into account the presence of m540 or apoptotic bodies
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متن کاملP-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies
Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...
متن کاملFlow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency
Background Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. FCM provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. During spermiogenesis histones are replaced by prota...
متن کاملflow cytometry: a new approach for indirect assessment of sperm protamine deficiency
background: flow cytometry (fcm) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. fcm provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. during spermiogenesis histones are replaced by prot...
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عنوان ژورنال
دوره 5 شماره 3
صفحات 128- 133
تاریخ انتشار 2011-12-01
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